Coopoosamy R, Interaction of Ziziphus mucronata subsp. mucronata Methanol.pdf (1.22 MB)
Interaction of ziziphus mucronata subsp. mucronata methanol extract and first-line antibiotics is synergistic In Vitro through production of reactive oxygen species
journal contribution
posted on 2022-01-06, 06:51 authored by Aderonke Ariyike Olajuyigbe, Olufunmiso Olusola Olajuyigbe, Roger Murugas CoopoosamyWith the increased incidence of antibacterial resistance in microorganisms, combining natural products from plants with
antibiotics may be considered interesting alternatives for synergy to attain multitarget effects. In this study, the antioxidant activity
of the methanol extract of Ziziphus mucronata and its interactions with antibiotics against bacteria of clinical importance were
investigated. While its phytochemicals and antioxidant activities were determined by free radical scavenging assays, the anti?bacterial activities of the extract and its interactions with the antibiotics were determined by macrobroth dilution and the
checkerboard methods. From the results, total phenolic content was 29.67 ± 1.90 mg GAE/100 g, total flavonoid content was
8.72 ± 0.08 mg QE/100 g, and total proanthocyanidin content was 1.94 ± 0.00 mg CE/100 g of dry plant material. *e inhibition
concentration 50% (IC50) of DPPH, BHT, and ascorbic acid was equal to 0.04 ± 0.02 mg/ml, respectively. *ose of the ABTS, BHT,
and ascorbic acid were equal to 0.02 ± 0.02, 0.04 ± 0.03, and 0.04 ± 0.02 mg/ml, respectively. *e checkerboard assay showed that
combining the extract with different antibiotics resulted in synergistic (38.75%), indifferent (30%), additive (28.75%), and
antagonistic (2.5%) interactions. *e interactions between the extract and antibiotics resulting in enhanced antibacterial activities
could have resulted from the antioxidant activities of the extract mopping up the ROS generated by the antibiotics or the ability of
both extract and antibiotics simultaneously producing reactive oxygen species with deleterious effects resulting in synergistic
antibacterial effects.